OB Cadherin anticorps (Intracellular)

N° du produit ABIN337035

Cet anticorps anti-OB Cadherin est un anticorps Souris Monoclonal détectant OB Cadherin dans WB, ELISA, IHC, IP, IHC (fro) et IHC (p). Adapté pour Humain et Souris.

Aperçu rapide pour OB Cadherin anticorps (Intracellular) (ABIN337035)

Antigène: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp OB Cadherin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5B2H5

Détail du produit anti-OB Cadherin anticorps

Épitope: Intracellular

Marque: IHC-plus™

Specificité: Recognizes the ~110kD human Cadherin 11 protein. Cross-reactivity with other cadherin family members has not been observed.

Purification: Protein A purified

Immunogène: Recombinant protein consisting of intracellular domain of human cadherin 11.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-Fr (5 - 10 μg/mL), IHC-P (5 - 10 μg/mL), IP (5 μg/mL), WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Immunoprecipitation: 5 μg. Native, human, Not tested for mouse. Positive control: MDA. MB231 cells (human) and mouse L fibroblast lysates.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Aliquot to Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.

Détails sur OB Cadherin

Antigène: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Autre désignation: CDH11 / Cadherin 11

Sujet: Name/Gene ID: CDH11
Family: Cadherin

Synonyms: CDH11, CAD11, Cadherin 11, Cadherin-11, CDHOB, OSF-4, Osteoblast cadherin, OB, OB-cadherin, Ventral neural cadherin, Vnc

ID gène: 1009

UniProt: P55287