Isocitrate Dehydrogenase anticorps

N° du produit ABIN337057

Cet anticorps Lapin Polyclonal détecte spécifiquement Isocitrate Dehydrogenase dans WB, ELISA, IHC et IHC (p). Il présente une réactivité envers Humain, Porc et Vicia faba.

Aperçu rapide pour Isocitrate Dehydrogenase anticorps (ABIN337057)

Antigène: Isocitrate Dehydrogenase (IDH)

Reactivité: Humain, Porc, Vicia faba

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Isocitrate Dehydrogenase est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Isocitrate Dehydrogenase anticorps

Specificité: Binds both Dehydroepiandrosterone (DHEA) and DHEAS, demonstrating minimal cross-reactivity with Progesterone and Testosterone. DHEA is the most abundant hormone in the human body and a precursor of all the sex hormones. It is synthesized principally in the adrenal glands and gonads from cholesterol, through conversion to pregnenolone and 17a-Hydroxypregnenolone, by the action of P450 enzymes. DHEA is mainly found in serum as the sulphated form (DHEAS), a reversible reaction catalyzed by sulfotransferase in the adrenal glands, liver and small intestine.

Purification: Affinity purified

Immunogène: Purified isocitrate dehydrogenase from porcine heart.

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (1:400 - 1:1600), IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Commentaires:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Store undiluted.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Détails sur Isocitrate Dehydrogenase

Antigène: Isocitrate Dehydrogenase (IDH)