FHL1 anticorps (AA 261-272)

N° du produit ABIN337221

Cet anticorps anti-FHL1 Polyclonal Chèvre (ABIN337221) détecte spécifiquement FHL1 dans WB, ELISA, IHC et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris, Rat et Hamster.

Aperçu rapide pour FHL1 anticorps (AA 261-272) (ABIN337221)

Antigène: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Reactivité: Humain, Souris, Rat, Hamster

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FHL1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-FHL1 anticorps

Épitope: AA 261-272

Specificité: Human FHL1. This antibody is expected to recognise isoforms 2( NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.

Homologie: Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%) Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Sheep, Elephant, Bovine, Dog, Horse, Rabbit, Pig, Xenopus (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide CNKRFVFHNEQVY from an internal region of human FHL1 (NP_001440.1, NP_001153171.1). Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%), Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Sheep, Elephant, Bovine, Dog, Horse, Rabbit, Pig, Xenopus (92%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.05 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur FHL1

Antigène: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Autre désignation: SLIM / FHL1

Sujet: Name/Gene ID: FHL1

Synonyms: FHL1, BA535K18.1, FHL1B, FHL1A, FLH1A, Four and a half LIM domains 1, FHL-1, KYOT, Skeletal muscle LIM-protein 1, SLIM-1, Skeletal muscle LIM-protein1, SLIMMER, XMPMA, KYO-T, LIM protein SLIMMER, SLIM, SLIM1

ID gène: 2273

NCBI Accession: NP_001440, NP_001153171

UniProt: Q13642