Thrombospondin 1 anticorps (Collagen-binding Domain)

N° du produit ABIN337292

L’anticorps Souris Monoclonal anti-Thrombospondin 1 a été validé pour WB, IHC, IP, IF, FACS et IHC (p). Il convient pour détecter Thrombospondin 1 dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Cheval, Porc, Chien et Mouton.

Aperçu rapide pour Thrombospondin 1 anticorps (Collagen-binding Domain) (ABIN337292)

Antigène: Thrombospondin 1 (THBS1)

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Cheval, Porc, Chien, Mouton

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Thrombospondin 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: A6-1

Détail du produit anti-Thrombospondin 1 anticorps

Épitope: Collagen-binding Domain

Marque: IHC-plus™

Specificité: Recognizes Thrombospondin. Shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild cross-reaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e. g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i. e. at low concentration of Ca2+. Species cross-reactivity: Human, bovine, porcine, equine, canine, sheep, mouse and rat.

Purification: Protein G purified

Immunogène: Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP.

Isotype: IgG1

Information d'application

Indications d'application: Approved: Flo, IF, IHC, IHC-P (10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4.

Agent conservateur: Azide free

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Thrombospondin 1

Antigène: Thrombospondin 1 (THBS1)

Autre désignation: Thrombospondin 1

Pathways: Autophagy