Thrombospondin 1 anticorps (Collagen-binding Domain)

N° du produit ABIN337292

Détail du produit anti-Thrombospondin 1 anticorps

Antigène: Thrombospondin 1 (THBS1)

Épitope: Collagen-binding Domain

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Cheval, Porc, Chien, Mouton

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Thrombospondin 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Recognizes Thrombospondin. Shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild cross-reaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e. g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i. e. at low concentration of Ca2+. Species cross-reactivity: Human, bovine, porcine, equine, canine, sheep, mouse and rat.

Purification: Protein G purified

Immunogène: Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP.

Clone: A6-1

Isotype: IgG1

Information d'application

Indications d'application: Approved: Flo, IF, IHC, IHC-P (10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4.

Agent conservateur: Azide free

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Thrombospondin 1

Antigène: Thrombospondin 1 (THBS1)

Autre désignation: Thrombospondin 1 (THBS1 Produits)

Synonymes: anticorps THBS, anticorps THBS-1, anticorps TSP, anticorps TSP-1, anticorps TSP1, anticorps Thbs-1, anticorps tbsp1, anticorps thrombospondin-1, anticorps thbs, anticorps thbs-1, anticorps tsp, anticorps tsp-1, anticorps tsp1, anticorps Tsp1, anticorps THBS1, anticorps Tsp-1, anticorps wu:fc48c03, anticorps si:dkey-11e23.1, anticorps tsp2, anticorps thrombospondin 1, anticorps thrombospondin 1 S homeolog, anticorps thrombospondin 1b, anticorps thrombospondin 2 L homeolog, anticorps THBS1, anticorps Thbs1, anticorps thbs1.S, anticorps thbs1b, anticorps thbs1, anticorps thbs2.L

Pathways: Autophagy