Complement C2 anticorps

N° du produit ABIN337358

L’anticorps Souris Monoclonal anti-Complement C2 a été validé pour WB, ELISA, IHC et IHC (p). Il convient pour détecter Complement C2 dans des échantillons de Humain.

Aperçu rapide pour Complement C2 anticorps (ABIN337358)

Antigène: Complement C2

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Complement C2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Complement C2 anticorps

Marque: IHC-plus™

Specificité: Recognizes C2 from human plasma/serum. Epitope specificity differs from that of LS-C22277 and LS-C22278 as determined by inhibition ELISA. Reacts strongly with C2. Strong reaction is seen with human serum/plasma and no reaction is seen with serum from patients deficient in C2, when tested in sandwich ELISA with a polyclonal antibody against C2. In ELISA, reaction is also seen with C2 directly coated onto the microtiter well.

Purification: Protein A/G purified

Immunogène: Complement component C2 isolated from human plasma.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA (1:2000), IHC, IHC-P (10 μg/mL), WB (1:125)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: After SDS-PAGE, this antibody reacts with C2 and a subfraction of C2 believed to be C2b, both under reducing and non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur Complement C2

Antigène: Complement C2

Sujet: Name/Gene ID: C2
Subfamily: Serine S1
Family: Protease

Synonyms: C2, C2b, C3/C5 convertase, Complement component C2, Complement C2, Complement component 2, CO2

ID gène: 717

UniProt: P06681