SMAD9 anticorps (AA 166-179)

N° du produit ABIN337365

Cet anticorps anti-SMAD9 Polyclonal Chèvre (ABIN337365) détecte spécifiquement SMAD9 dans WB, ELISA, IHC et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Chien, Roussette (Chauve-souris), Poulet, Boeuf (Vache), Hamster, Cheval, Singe, Porc et Lapin.

Aperçu rapide pour SMAD9 anticorps (AA 166-179) (ABIN337365)

Antigène: SMAD9 (SMAD Family Member 9 (SMAD9))

Reactivité: Humain, Souris, Rat, Chien, Roussette (Chauve-souris), Poulet, Boeuf (Vache), Hamster, Cheval, Singe, Porc, Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD9 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-SMAD9 anticorps

Épitope: AA 166-179

Specificité: Human SMAD9. This antibody is not expected to cross-react with SMAD1 and SMAD5.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Turkey, Chicken (100%) Opossum, Platypus, Lizard (93%) Stickleback (86%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-HSEPLMPHNATYPD from an internal region of human SMAD9 (NP_005896.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Turkey, Chicken (100%), Opossum, Platypus, Lizard (93%), Stickleback (86%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur SMAD9

Antigène: SMAD9 (SMAD Family Member 9 (SMAD9))

Autre désignation: SMAD9

Sujet: Name/Gene ID: SMAD9

Synonyms: SMAD9, MAD homolog 9, SMAD 9, SMAD8A, SMAD8, Smad9, MADH9, SMAD8B, MADH6, Mothers against DPP homolog 9, SMAD family member 9

ID gène: 4093

NCBI Accession: NP_005896

UniProt: O15198