PAR1 anticorps (N-Term)

N° du produit ABIN337370

L’anticorps Souris Monoclonal anti-PAR1 a été validé pour ELISA, IHC, FACS, IF et IHC (p). Il convient pour détecter PAR1 dans des échantillons de Humain.

Aperçu rapide pour PAR1 anticorps (N-Term) (ABIN337370)

Antigène: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PAR1 est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ATAP2

Détail du produit anti-PAR1 anticorps

Épitope: N-Term

Marque: IHC-plus™

Specificité: Recognizes the cleaved and intact forms of human thrombin receptor PAR1, N-terminus. Does not cross-react with PAR2.

Purification: Protein A purified

Immunogène: A synthetic peptide corresponding to the N-terminal region of the human thrombin receptor PAR1.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Information d'application

Indications d'application: Approved: ELISA (0.1 - 1 μg/mL), Flo (10 - 20 μg/mL), IF (1 - 3 μg/mL), IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Immunofluorescence (Methanol or acetone fixed CHRF cells): 1-3 μg/mL. Flow Cytometry (Methanol or acetone fixed CHRF cells): 10-20 μg/mL. ELISA: 0.1-1.0 μg/mL.

Not recommended for: WB

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur PAR1

Antigène: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Autre désignation: F2R / Thrombin Receptor / PAR1

Sujet: Name/Gene ID: F2R
Subfamily: Proteinase-activated
Family: GPCR

Synonyms: F2R, CF2R, Coagulation factor II receptor, PAR1, PAR-1, Thrombin receptor, TR, HTR, Protease-activated receptor 1

ID gène: 2149

UniProt: P25116

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus