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AKT Substrate (pSer), (pThr) anticorps

Cet anticorps anti- est un anticorps Lapin Polyclonal détectant dans ELISA, WB, IHC (p) et IP. Adapté pour .
N° du produit ABIN337521

Aperçu rapide pour AKT Substrate (pSer), (pThr) anticorps (ABIN337521)

Antigène

AKT Substrate

Reactivité

Veuillez nous consulter SVP

Hôte

  • 2
Lapin

Clonalité

  • 2
Polyclonal

Conjugué

  • 2
Inconjugué

Application

ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
  • Épitope

    pSer, pThr

    Specificité

    Preferentially recognizes peptides and proteins containing phospho-Ser/Thr preceded by Lys/Arg at positions -5 and -3, in a manner largely independent of other surrounding amino acids. Some cross-reactivity is observed for peptides that contain phospho-Ser/Thr preceded by Arg/Lys at positions -3 and -2. No cross-reactivity is observed with the corresponding non-phosphorylated sequences or with other phospho-Ser/Thr-containing motifs. By ELISA the antibody recognizes a wide range of phosphorylated Akt substrate peptides and by 2D gel Western blot analysis it recognizes a large number of presumptive Akt substrate proteins.

    Purification

    Immunoaffinity Chromatography

    Immunogène

    Synthetic phospho- Akt substrate peptides.

    Isotype

    IgG
  • Indications d'application

    ELISA (1:1000), IHC-P, IP (1:20), WB (1:1000)

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, BSA, 50 % glycerol.

    Conseil sur la manipulation

    avoid freeze thaw cycles. Store undiluted.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
  • Antigène

    AKT Substrate

    Classe de substances

    Chemical
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