Luciferase anticorps

N° du produit ABIN339711

Cet anticorps anti-Luciferase est un anticorps Souris Monoclonal détectant Luciferase dans WB, IF, IHC et IHC (fro). Adapté pour Photinus pyralis.

Aperçu rapide pour Luciferase anticorps (ABIN339711)

Antigène: Luciferase

Reactivité: Photinus pyralis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Luciferase est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Luciferase anticorps

Specificité: This antibody specifically reacts with luciferase protein, a reporter used to quantify expression levels from promoters in tissue culture and in vivo. Reactivity was confirmed via Western Blot analysis on the following samples containing added purified luciferase protein: mouse heart, liver, kidney, and cortex tissues. There was no cross reactivity to mouse heart, NIH3T3, Jurkat, and human fibroblasts. In IHC (frozen tissue) and Immunofluorescence applications, our antibody was able to detect tissue specific expression of Luciferase protein in Drosophila embryos using the well-characterized Gal4-UAS system with two different Gal4 drivers.

Purification: Protein A purified

Immunogène: Luciferase protein from Photinus pyralis.

Type of Immunogen: Purified protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: IF (10 - 30 μg/mL), IHC, IHC-Fr, WB (1 - 3 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Western Blot: 1-3 μg/mL. Observed in mouse heart, liver, kidney, and cortex tissues containing added purified luciferase protein. Immunohistochemistry (Frozen Tissue): 15-50 μg/mL. Detects tissue specific expression of Luciferase protein in Drosophila embryos using Gal4-UAS system with two different Gal4 drivers. Immunofluorescence: 10-30 μg/mL.

Commentaires:

Target Species of Antibody: Photinus pyralis

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Luciferase

Antigène: Luciferase

Autre désignation: Firefly Luciferase

Sujet: Synonyms: Firefly Luciferase, PPLUC

UniProt: P08659