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Phosphothreonine anticorps

L’anticorps Lapin Polyclonal anti-Phosphothreonine a été validé pour WB, IP et ELISA. Il convient pour détecter Phosphothreonine dans des échantillons de .
N° du produit ABIN340103

Aperçu rapide pour Phosphothreonine anticorps (ABIN340103)

Antigène

Phosphothreonine

Reactivité

Veuillez nous consulter SVP

Hôte

  • 16
  • 6
Lapin

Clonalité

  • 15
  • 7
Polyclonal

Conjugué

  • 13
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Cet anticorp Phosphothreonine est non-conjugé

Application

  • 22
  • 19
  • 15
  • 7
  • 6
  • 5
  • 5
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP), ELISA
  • Specificité

    Recognizes phosphothreonine, peptidyl-phosphothreonine and threonine-phosphorylated proteins. Species cross-reactivity: species independent.

    Purification

    Immunoaffinity purified

    Immunogène

    Phosphothreonine conjugated to KLH and phosvitin.

    Type of Immunogen: Hapten

    Isotype

    IgG
  • Indications d'application

    Approved: ELISA (0.5 μg/mL), IP, WB (2 μg/mL)

    Usage: Suitable for use in Western Blot, ELISA, and Immunoprecipitation. Western Blot: 2 μg/mL, Mouse brain lysate. Incubate at RT for 4 hrs. ELISA: 0.5 μg/mL (kinase assay). Binding of Ly-pT-Gly-HRP. (0.2 μg/100 μL/well) to an anti-phosphothreonine coated microplate (0.5 μg/100 μL/well) is inhibited by 95 % with the addition of 2-10 μg/mL anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1 mg/mL.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7, 0.01 % sodium azide, 50 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Antigène

    Phosphothreonine

    Classe de substances

    Amino Acid
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