L’anticorps Lapin Polyclonal anti-Phosphothreonine a été validé pour WB, IP et ELISA. Il convient pour détecter Phosphothreonine dans des échantillons de .
N° du produit ABIN340103
Aperçu rapide pour Phosphothreonine anticorps (ABIN340103)
Antigène
Phosphothreonine
Reactivité
Veuillez nous consulter SVP
Hôte
Lapin
Clonalité
Polyclonal
Conjugué
Cet anticorp Phosphothreonine est non-conjugé
Application
Western Blotting (WB), Immunoprecipitation (IP), ELISA
Specificité
Recognizes phosphothreonine, peptidyl-phosphothreonine and threonine-phosphorylated proteins. Species cross-reactivity: species independent.
Usage: Suitable for use in Western Blot, ELISA, and Immunoprecipitation. Western Blot: 2 μg/mL, Mouse brain lysate. Incubate at RT for 4 hrs. ELISA: 0.5 μg/mL (kinase assay). Binding of Ly-pT-Gly-HRP. (0.2 μg/100 μL/well) to an anti-phosphothreonine coated microplate (0.5 μg/100 μL/well) is inhibited by 95 % with the addition of 2-10 μg/mL anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1 mg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
PBS, pH 7, 0.01 % sodium azide, 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.