West Nile Virus anticorps

N° du produit ABIN340476

Cet anticorps anti-West Nile Virus est un anticorps Souris Monoclonal détectant West Nile Virus dans ELISA, WB, IP et Neut. Adapté pour West Nile Virus (WNV).

Aperçu rapide pour West Nile Virus anticorps (ABIN340476)

Antigène: West Nile Virus (WNV)

Reactivité: West Nile Virus (WNV)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp West Nile Virus est non-conjugé

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP), Neutralization (Neut)

Clone: 345712

Détail du produit anti-West Nile Virus anticorps

Specificité: Recognizes West Nile Virus NS2b subunit.

Purification: Protein G purified

Immunogène: E. coli-derived rwnvNS3/NS2b.

Type of Immunogen: Recombinant protein

Isotype: IgG3

Information d'application

Indications d'application: Approved: ELISA, IP, Neut, WB

Usage: Western Blot, Neutralization, Immunoprecipitation, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect west nile virus NS3/NS2b complex. Using a colorimetric detection system, the detection limit for rwnvNS3/NS2b complex is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Neutralization of west nile virus NS3 bioactivity: This antibody has been used to inhibit/neutralize the enzymatic activity of rwnvNS3/NS2b. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 min. The enzyme was then assayed with a peptide substrate, Ac-REKR-AFC. 50 % of the proteolytic activity was inhibited by the antibody at approximately 13 μg/mL (IC50) under conditions in which the enzyme was present at 10 μg/mL and the substrate concentration was 200uM. Considering the molecular masses of the enzyme (27kD) and the antibody (150kD), IC50 was achieved at approximately 1:4 Molar ratio of the antibody to the enzyme. Immunoprecipitation: This antibody was used at a concentration of 25 μg/mL to immunoprecipitate rwnvNS3/NS2b from conditioned cell culture medium. The recovered rwnvNS3/NS2b can be detected in Western blots using a polyclonal antibody. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect west nile virus NS3/NS2b complex. The detection limit for rwnvNS3/NS2b is approximately 1 ng/well.

Commentaires:

Target Species of Antibody: West Nile Virus

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution: Reconstitute, sterile TBS. If 1 mL of TBS is used, the antibody concentration will be 100 μg/mL.

Concentration: Lot specific

Buffer: Lyophilized from TBS, 5 % trehalose. Reconstitution: Reconstitute, sterile TBS. If 1 mL of TBS is used, the antibody concentration will be 100 μg/mL.

Détails sur West Nile Virus

Antigène: West Nile Virus (WNV)

Autre désignation: West Nile Virus

Classe de substances: Virus