Phosphothreonine-PROLINE anticorps

N° du produit ABIN344517

Cet anticorps anti-Phosphothreonine-PROLINE Monoclonal Souris (ABIN344517) détecte spécifiquement Phosphothreonine-PROLINE dans WB, ELISA, IHC et IHC (p).

Aperçu rapide pour Phosphothreonine-PROLINE anticorps (ABIN344517)

Antigène: Phosphothreonine-PROLINE

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit

Specificité: Detects phospho- threonine only when followed by proline. It reacts with proteins/peptides phosphorylated on the Thr-Pro motif in an otherwise highly context-independent fashion. The phosphospecific antibody does not recognize phospho-threonine in the absence of an adjacent proline. Does not react with phospho-tyrosine, but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e. g., phospho-Elk-1). Recognizes all species.

Purification: Protein A purified

Immunogène: Synthetic phosphothreonine- proline-containing peptides (KLH coupled). Antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody.

Type of Immunogen: Synthetic peptide

Isotype: IgM

Information d'application

Indications d'application: Approved: ELISA (1:1000), IHC, IHC-P, WB (1:5000)

Usage: Western Blot: 1:5000. Immunohistochemistry (Paraffin): 1:50. ELISA: 1:1000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Agent conservateur: Without preservative

Détail du antigène

Antigène: Phosphothreonine-PROLINE

Autre désignation: Phospho-Threonine-Proline

Classe de substances: Chemical