COL2 anticorps

N° du produit ABIN344746

Cet anticorps Souris Monoclonal détecte spécifiquement COL2 dans WB, IHC, ELISA et IHC (p). Il présente une réactivité envers Humain, Souris et Poulet.

Aperçu rapide pour COL2 anticorps (ABIN344746)

Antigène: COL2 (Collagen, Type II (COL2))

Reactivité: Humain, Souris, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp COL2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-COL2 anticorps

Specificité: Recognizes both alpha 1 (II) and alpha 3 (XI) chains which have identical primary structure. It also reacts with pepsin-digested type II collagen. Its epitope is localized in the triple helix of type II collagen. It shows no cross-reaction with type I or type III collagen. Species cross-reactivity: Human, Chicken, Mouse, Salamander.

Purification: Purified

Immunogène: Purified type II collagen, obtained from adult chicken sternum by limited pepsinization.

Type of Immunogen: Purified protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-P (1:200), WB (1:200 - 1:400)

Usage: In Western blotting, antibody reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. Western Blot of CNBr peptides of collagen II shows that this antibody reacts with CB11 fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule. ELISA. Western Blot: 1:200-1:400. Cartilage is an appropriate positive control. Immunohistochemistry (Formalin/Paraffin): 1-2 μg/mL. Staining of paraffin embedded tissues requires digestion of tissue sections with pepsin at 1 mg/mL Tris HCl,  pH 2 for 15 minutes at RT or 15 minutes at 37?C.

Commentaires:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4, 0.2 % BSA, 15 mM sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur COL2

Antigène: COL2 (Collagen, Type II (COL2))

Autre désignation: Collagen Type II