LacI anticorps

N° du produit ABIN349605

Détail du produit

Antigène: LacI

Reactivité: E. coli, Shigella sonnei

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA

Specificité: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence. Cross-reactivity with Lac I from other sources has not been determined.

Réactivité croisée: Shigella Sonnei (S. sonnei)

Attributs du produit: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase, modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.

Purification: affinity purified

Stérilité: Sterile filtered

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant Lac I protein.
Immunogen Type: RecombinantProtein

Isotype: IgG

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Lac I protein.   Specific conditions for reactivity and detection of Lac I should be optimized by the end user.  Expect a band approximately ~38 kDa in size corresponding to Lac I by western blotting in the appropriate cell lysate or extract.

Commentaires:

Gene Name: LAC1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 3 months

Référence

Chang, Thrall, Laureti, Piatt, Pagès, Loparo: "Compartmentalization of the replication fork by single-stranded DNA-binding protein regulates translesion synthesis." dans: Nature structural & molecular biology, Vol. 29, Issue 9, pp. 932-941, (2022) (PubMed).

Kuhn, Pascual-Garcia, Gozalo, Little, Capelson: "Chromatin targeting of nuclear pore proteins induces chromatin decondensation." dans: The Journal of cell biology, Vol. 218, Issue 9, pp. 2945-2961, (2020) (PubMed).

Wyatt, Xiao, Americo, Earl, Moss: "Novel Nonreplicating Vaccinia Virus Vector Enhances Expression of Heterologous Genes and Suppresses Synthesis of Endogenous Viral Proteins." dans: mBio, Vol. 8, Issue 3, (2018) (PubMed).

Agelopoulos, McKay, Mann: "Developmental regulation of chromatin conformation by Hox proteins in Drosophila." dans: Cell reports, Vol. 1, Issue 4, pp. 350-9, (2013) (PubMed).

Détail du antigène

Antigène: LacI

Autre désignation: Lac I

Synonymes: anticorps ECK0342, anticorps JW0336, anticorps lactose-inducible lac operon transcriptional repressor, anticorps lacI

Sujet: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase; modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.
Synonyms: DNA binding transcriptional repressor antibody, LacI antibody, Transcriptional repressor of the lac operon antibody

ID gène: 8156885, 146576

UniProt: P03023