PCK1 anticorps

N° du produit ABIN349649

Détail du produit anti-PCK1 anticorps

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivité: Zea mays, Arabidopsis thaliana, Orge, Nicotiana tabacum, Phaeodactylum tricornutum, Synechocystis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PCK1 est non-conjugé

Application: Western Blotting (WB)

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 110 / 105 kDa

Purification: serum

Immunogène: KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0, At1g53310 (PEPC 1), Q84VW9, At1g14940 (PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzyme NP_177043.2 (PEPC 4)

Information d'application

Indications d'application: Recommended Dilution: 1 : 1000 with standard ECL (WB).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Buffer: PBS pH 7.4

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Références pour anticorps anti-PCK1 (ABIN349649)

Chang, Jeon, Gu, Pack, Jin: "Conversion of carbon dioxide to oxaloacetate using integrated carbonic anhydrase and phosphoenolpyruvate carboxylase." dans: Bioprocess and biosystems engineering, (2013) (PubMed).

Aragón, Pascual, González, Escalona, Carvalho, Amancio: "The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles." dans: Plant cell reports, (2013) (PubMed).

Wostrikoff, Clark, Sato, Clemente, Stern: "Ectopic expression of Rubisco subunits in maize mesophyll cells does not overcome barriers to cell type-specific accumulation." dans: Plant physiology, Vol. 160, Issue 1, pp. 419-32, (2012) (PubMed).

Détails sur PCK1

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Autre désignation: Phosphoenolpyruvate Carboxylase (PCK1 Produits)

Sujet: AGI Code: At1g53310, At1g14940
PEPC (phosphoenolpyruvate carboxylase), EC=4.1.1.31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate. Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3

Poids moléculaire: expected: 110 kDa, apparent: 105 kDa

NCBI Accession: NP_177043

UniProt: Q84VW9, Q9MAH0

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis