Actin anticorps (C-Term)
Aperçu rapide pour Actin anticorps (C-Term) (ABIN350120)
Antigène
Voir toutes Actin AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- C-Term
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Fonction
- Sheep antibody to Actin
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Specificité
- Specific for Actin.
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Réactivité croisée
- Humain, Souris, Rat
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Réactivité croisée (Details)
- Other species not yet tested.
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Purification
- IgG
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Immunogène
- A synthetic peptide from the c-terminal of human beta gamma kappa Actin (ACTB ACTG ACTK) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many species including rat mouse zebra fish and xenopus.
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Isotype
- IgG
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Indications d'application
- IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
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Conseil sur la manipulation
- Avoid freeze and thaw cycles.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
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Date de péremption
- 12 months
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- Actin
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Sujet
- FUNCTION: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. SUBCELLULAR LOCATION: Cytoplasm cytoskeleton.
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UniProt
- P60709
Antigène
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