Cet anticorps Mouton Polyclonal détecte spécifiquement GFP dans WB et IHC. Il présente une réactivité envers Aequorea victoria et a été mentionné dans 1 publication.
GFP
Reactivité: Aequorea victoria
WB, ELISA, IHC, IP, FM, FACS
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
IHC WB. Use at a concentration of 10-50 μg,ml. The optimal working concentration should be determined by the end user.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Conseil sur la manipulation
Avoid freeze and thaw cycles.
Stock
4 °C,-20 °C
Stockage commentaire
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Date de péremption
12 months
Choi, Kim, Song, Cha, Cho, Son, Kim, Mook-Jung: "Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death." dans: Aging cell, Vol. 18, Issue 1, pp. e12872, (2020) (PubMed).
Antigène
GFP
(Green Fluorescent Protein (GFP))
Sujet
GFP is anaturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins.