ADAM12 anticorps (AA 2-14)

N° du produit ABIN351478

Cet anticorps Chèvre Polyclonal détecte spécifiquement ADAM12 dans ELISA, WB, IHC et IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour ADAM12 anticorps (AA 2-14) (ABIN351478)

Antigène: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ADAM12 est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ADAM12 anticorps

Épitope: AA 2-14

Specificité: Human ADAM12. This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS.

Homologie: Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Monkey (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide AARPLPVSPARALC from the N-terminus of human ADAM12 (NP_003465.3, NP_067673.2). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Monkey (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:64000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur ADAM12

Antigène: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Autre désignation: ADAM12

Sujet: Name/Gene ID: ADAM12
Subfamily: Metallopeptidase M12B
Family: Protease

Synonyms: ADAM12, ADAM 12, MCMPMltna, MCMP, MLTN, Meltrin-alpha, MLTNA

ID gène: 8038

NCBI Accession: NP_003465, NP_067673

UniProt: O43184

Pathways: EGFR Signaling Pathway