Amphiphysin anticorps (AA 159-170)

N° du produit ABIN351482

L’anticorps anti-Amphiphysin Polyclonal Chèvre est utilisé pour la détection de Amphiphysin dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Singe, Chien, Cheval, Lapin, Hamster et Roussette (Chauve-souris). Il a été validé pour WB, ELISA, IHC et IHC (p).

Aperçu rapide pour Amphiphysin anticorps (AA 159-170) (ABIN351482)

Antigène: Amphiphysin (AMPH)

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Singe, Chien, Cheval, Lapin, Hamster, Roussette (Chauve-souris)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Amphiphysin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Amphiphysin anticorps

Épitope: AA 159-170

Specificité: Human AMPH / Amphiphysin. This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit (100%) Xenopus (92%) Opossum, Chicken, Lizard (83%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-KRKDESRISKAE from an internal region of human AMPH / Amphiphysin (NP_001626.1, NP_647477.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit (100%), Xenopus (92%), Opossum, Chicken, Lizard (83%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000), WB (0.1 - 0.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Amphiphysin

Antigène: Amphiphysin (AMPH)

Autre désignation: AMPH / Amphiphysin

Sujet: Name/Gene ID: AMPH

Synonyms: AMPH, AMPH1, Amphiphysin, Amphiphysin I

ID gène: 273

NCBI Accession: NP_001626, NP_647477

UniProt: P49418