GFAP anticorps

N° du produit ABIN351523

Cet anticorps Souris Monoclonal détecte spécifiquement GFAP dans WB, IHC, IF, IHC (p) et IP. Il présente une réactivité envers Humain, Rat, Porc et Poulet.

Aperçu rapide pour GFAP anticorps (ABIN351523)

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivité: Humain, Rat, Porc, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFAP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Détail du produit anti-GFAP anticorps

Marque: IHC-plus™

Specificité: Recognizes porcine glial fibrillary acidic protein (GFAP) at 51-52kD. Species cross-reactivity: human, rat and chicken.

Purification: Protein G purified

Immunogène: Porcine spinal cord. Cellular Localization: Cytoplasmic.

Isotype: IgG1

Information d'application

Indications d'application: Approved: IF, IHC, IHC-P (1 - 2 μg/mL), IP (2 μg/tst), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1-2 μg/mL. Immunoprecipitation (Native and denatured): 2 μg/mg protein lysate (Use Protein G). Western Blot: 1-2 μg/mL for 2 hrs at RT. Positive control: Astrocytoma, IMR5 and brain cells.

Commentaires:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide, 40 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur GFAP

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Autre désignation: GFAP

Sujet: Name/Gene ID: GFAP

Synonyms: GFAP

ID gène: 2670

UniProt: P14136