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EpCAM anticorps

L’anticorps Souris Monoclonal anti-EpCAM a été validé pour FACS, IF, IHC (p) et IHC (fro). Il convient pour détecter EpCAM dans des échantillons de Humain.
N° du produit ABIN356312

Aperçu rapide pour EpCAM anticorps (ABIN356312)

Antigène

Voir toutes EpCAM (EPCAM) Anticorps
EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité

  • 372
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Humain

Hôte

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Souris

Clonalité

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Monoclonal

Conjugué

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Cet anticorp EpCAM est non-conjugé

Application

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

ESA214
  • Specificité

    This antibody has been used recently to characterize the EpCAM high CD44+ versus EpCAM low/Cd44- indifferent cancer cells.
    Cellular Localization: Cell membrane.

    Attributs du produit

    Synonyms: Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2,M4S1, MIC18, Tumor-associated calcium signal transducer 1, Major gastrointestinaltumor-associated protein GA733-2, Epithelial glycoprotein 314,Adenocarcinoma-associated antigen, KS 1/4 antigen

    Purification

    Protein A Chromatography.

    Immunogène

    Small cell lung carcinoma. Remarks: Molecular weight of antigen: 40 kDa.

    Isotype

    IgG1
  • Indications d'application

    Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. Antigen retriever like pepsin enhances the staining of FFPE tissue. Immunofluorescence. Flow Cytometry. Recommended Positive Control: Breast carcinoma.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    0.2 mg/mL

    Buffer

    PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: One year from despatch.

    Date de péremption

    12 months
  • Antigène

    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

    Autre désignation

    CD326 / EPCAM / TACSTD1

    Sujet

    Epithelial is a transmembrane glycoprotein (40 kDa) also known as ESA or epithelial cellular adhesion molecule (Ep-CAM). This protein is expressed on basolateral cell surface in most simple epithelia and vast majority of cancers. According to the Synonyms: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1

    ID gène

    4072

    UniProt

    P16422
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