L’anticorps anti-CA 19-9 Monoclonal Souris est utilisé pour la détection de CA 19-9 dans des échantillons de Humain. Il a été validé pour IHC (p) et IHC (fro).
CA 19-9
Reactivité: Humain
FACS, IF, IHC (p), WB
Hôte: Lapin
Monoclonal
CA19-9-1390RCA19-9-1390R
unconjugated
Recombinant Antibody
Indications d'application
Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. FFPE tissue section requires antigen retriever (boiling tissue in 10 mM citrate, pH 6.0 for 20 mins, followed by cooling at RT for 20 mins). Recommended Positive Control: Human colon, stomach, pancreas, intestine, breastcarcinomas. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.2 mg/mL
Buffer
PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Date de péremption
12 months
Antigène
CA 19-9
(Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))
Autre désignation
CA 19-9
Sujet
The CA 19-9 antigen is a monosialosyl Le (a) blood group antigen which has been shown to be a useful-associated antigen for the diagnosis of gastrointestinal cancers. This antigen is known to be a ligand for the cell adhesion molecule called ELAM-1 (E-selectin, endothelial cell leukocyte adhesion molecule-1, which is present on cytokine-activated human endothelial cells. Selectin GMP-140 (CD62,PADGEM) binds to sialosyl Le (a) and sulfated glycans modulate this binding.Synonyms: CA19-9, Sialosyl Lewis a, Sialyl Lewis a