CD175s anticorps

N° du produit ABIN356328

Cet anticorps anti- est un anticorps Souris Monoclonal détectant dans FACS, IHC (fro), IF et IHC (p). Adapté pour Humain. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour CD175s anticorps (ABIN356328)

Antigène: CD175s

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: STN218

Détail du produit

Attributs du produit: Synonyms: Sialosyl Tn, Sialosyl-Tn, Sialyl Tn, sTn, NeuAc a GalNAc

Purification: Purified

Immunogène: Ovine submaxillary mucin (OSM)Remarks: Molecular weight of antigen: Several HM weight glycoproteins. Epitope: NeuAc alphaGalNacOSer/Thr.

Isotype: IgG1

Information d'application

Indications d'application: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. Immunofluorescence. Flow Cytometry. Recommended Positive Control: Human gastrointestinal tumor, prostate, ovary ca
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Date de péremption: 12 months

Référence

Hofmann, Schlüter, Lange, Mercanoglu, Ewald, Fölster, Picksak, Harder, El Gammal, Grupp, Güngör, Drenckhan, Schlüter, Wagener, Izbicki, Jücker, Bockhorn, Wolters-Eisfeld: "COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer." dans: Molecular cancer, Vol. 14, pp. 109, (2015) (PubMed).

Détail du antigène

Antigène: CD175s

Sujet: Sialosyl Tn antigen also known as CD175s is expressed in the majority of colon, gastric, breast and lung carcinoma. The sialosyl Tn has limited expression to being absent in normal colon, pancreas, stomach, ovary, and prostate. This antigen is detected in several adenocarcinomas. It appears to be useful marker for poorly differentiated colon adenocarcinomas. This antibody stains colon carcinomas and ovarian mucinous carcinomas producing apical cytoplasmic, apical membrane or luminal staining patterns. It is negative with majority of adult tissue sections.Synonyms: NeuAc a GalNAc, Sialosyl Tn, Sialosyl-Tn, Sialyl Tn, sTn