Fibronectin anticorps

N° du produit ABIN356452

Cet anticorps anti-Fibronectin Polyclonal Lapin (ABIN356452) détecte spécifiquement Fibronectin dans IHC (p) et IHC (fro). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Fibronectin anticorps (ABIN356452)

Antigène: Fibronectin

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Fibronectin est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Fibronectin anticorps

Specificité: This antibody reacts with Human Fibronectin in liver, tonsil, skin and kidney. Cellular localization: Connective tissue matrix and basement membrane.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography on Protein A

Immunogène: Purified human Fibronectin from human plasma.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry: 1/100-1/400 for 30 minute at room temp. using polymer system orstreptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections andcell smears require antigen retriever, boiling tissue in 10 mM citrate buffer, pH 6.0 for 10-20 minute, followed by cooling at room temp. for 20 minutes. Positive control: Human kidney.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 2.0 mg/mL

Buffer: 10 mM PO4, 150 mM NaCl, pH 7.4, 0.05 % Sodium Azide, 1 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur Fibronectin

Antigène: Fibronectin

Sujet: Fibronectin is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and extracellular matrix. Fibronectin is involved in cell adhesion and migration process including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. They also occur as dimmer of two 200 kDa subunits. Fibronectin has been implicated in carcinoma development in lung cancer. Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents.Synonyms: CIG, Cold-insoluble globulin, FN1

ID gène: 2335

NCBI Accession: NP_002017

UniProt: P02751