ERAS anticorps (N-Term)
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- Antigène Voir toutes ERAS Anticorps
- ERAS (ES cell expressed Ras (ERAS))
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Épitope
- N-Term
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp ERAS est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificité
- This antibody detects ERAS at N-term.
- Purification
- Protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Immunogène
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ERAS.
- Isotype
- Ig Fraction
- Top Product
- Discover our top product ERAS Anticorps primaire
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- Indications d'application
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ELISA 1: 1,000. Western blot 1: 50 - 1: 100. Immunofluorescence 1: 10 - 1: 50. Immunohistochemistry 1: 50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- PBS with 0.09 % (W/V) sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- Antigène
- ERAS (ES cell expressed Ras (ERAS))
- Autre désignation
- ERAS / HRAS2 (ERAS Produits)
- Synonymes
- anticorps HRAS2, anticorps HRASP, anticorps HRasp, anticorps Ha-Ras2, anticorps ecat5, anticorps ES cell expressed Ras, anticorps ES cell-expressed Ras, anticorps ERAS, anticorps Eras
- Sujet
- Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.Synonyms: E-Ras, Embryonic stem cell-expressed Ras, GTPase ERas, HRASP
- Poids moléculaire
- 25156 Da
- ID gène
- 3266, 9606
- UniProt
- Q7Z444
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