Osteocalcin anticorps (N-Term)

N° du produit ABIN357367

Cet anticorps Lapin Polyclonal détecte spécifiquement Osteocalcin dans WB, IHC (p) et EIA. Il présente une réactivité envers Humain.

Aperçu rapide pour Osteocalcin anticorps (N-Term) (ABIN357367)

Antigène: Osteocalcin (BGLAP)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Osteocalcin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-Osteocalcin anticorps

Épitope: AA 9-39, N-Term

Specificité: This antibody detects Osteocalcin at N-term.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogène: Synthetic peptide KLH conjugated between 9~39 amino acids from the N-terminal region of Human Osteocalcin.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur Osteocalcin

Antigène: Osteocalcin (BGLAP)

Autre désignation: Osteocalcin

Sujet: Prior to the formation of calcified bone, noncollagenous proteins form in the extracellular bone matrix. Gamma-carboxyglutamic acid residues are formed by vitamin K, vitamin-D regulated calcium binding proteins containing residues of Gla. These residues are essential for the binding of calcium and constitue 1-2 % of total bone protein. Osteocalcin itself binds strongly to apatite and calcium. Production of osteocalcin is expressed late in normal bone development and is characteristic of mature osteoblasts. Regular osteocalcin production has been shown to be linked to the p53 tumor suppressor gene. The p53 gene undergoes rearrangement in a high percentage of osteosarcomas, resulting in loss of its expression. The loss of p53 regulation inhibits further osteocalcin production. The absence of end-point differentiation in bone due to p53 rearrangements and lack of osteocalcin production may contribute to the maintenance of the tumorigenic phenotype in osteosarcomas.Synonyms: BGLAP, Bone Gla protein, Gamma-carboxyglutamic acid-containing protein

Poids moléculaire: 10962 Da

ID gène: 632

NCBI Accession: NP_954642

UniProt: P02818