PKMYT1 anticorps (pThr495)

N° du produit ABIN358168

Cet anticorps Lapin Polyclonal détecte spécifiquement PKMYT1 dans WB, IHC (p) et EIA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour PKMYT1 anticorps (pThr495) (ABIN358168)

Antigène: PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKMYT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-PKMYT1 anticorps

Épitope: pThr495

Specificité: This antibody detects MYT1 pThr495.

Purification: Protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogène: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T495 of human MYT1.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA: 1/1,000. Western Blot: 1/100-1/500. Dot Blot: 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur PKMYT1

Antigène: PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))

Autre désignation: PKMYT1

Sujet: The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported.Synonyms: MYT1, Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase

Poids moléculaire: 54521 Da

ID gène: 9088, 9606

UniProt: Q99640

Pathways: Mitotic G1-G1/S Phases, M Phase