LEFTY1 anticorps

N° du produit ABIN360926

L’anticorps anti-LEFTY1 Monoclonal Rat est utilisé pour la détection de LEFTY1 dans des échantillons de Souris. Il a été validé pour WB.

Aperçu rapide pour LEFTY1 anticorps (ABIN360926)

Antigène: LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp LEFTY1 est non-conjugé

Application: Western Blotting (WB)

Clone: 8B65

Détail du produit anti-LEFTY1 anticorps

Specificité: This antibody detects Lefty-1.

Purification: Protein A/G affinity chromatography

Immunogène: Recombinant protein of Lefty-1

Isotype: IgG2

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 200 μL sterile PBS and the final concentration is 500 μg/mL.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur LEFTY1

Antigène: LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))

Autre désignation: LEFTY1

Sujet: During vertebrate embryogenesis, a left right axis is established. Secreted growth factors of the TGF beta family, including gene products derived from nodal, lefty 1 and lefty 2, play crucial roles in establishing left right asymmetries. TGF beta (Transforming growth factor beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF beta actions are directed by ligand induced activation of TGF beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF beta superfamily, inhibits TGF beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF beta receptor. Lefty also inhibits events downstream from R Smad phosphorylation. Lefty provides a repressed state of TGF beta responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (41 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28 kDa form induces MAPK activity.Synonyms: LEFTB, LEFTY1, LEFTYB, Left-right determination factor 1, Left-right determination factor B, Protein lefty-1, Protein lefty-B

ID gène: 13590

NCBI Accession: NP_034224

UniProt: Q64280