XPNPEP1 anticorps

N° du produit ABIN360952

Détail du produit anti-XPNPEP1 anticorps

Antigène: XPNPEP1 (X-Prolyl Aminopeptidase (Aminopeptidase P) 1, Soluble (XPNPEP1))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp XPNPEP1 est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: This antibody reacts to Aminopeptidase P.

Purification: Protein G chromatography

Immunogène: BALB/c mice using a membrane protein fraction of isolated rat glomeruli

Clone: JG12C9C10

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry on frozen and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.

Buffer: PBS pH 6,0

Précaution d'utilisation: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur XPNPEP1

Antigène: XPNPEP1 (X-Prolyl Aminopeptidase (Aminopeptidase P) 1, Soluble (XPNPEP1))

Autre désignation: XPNPEP1 (XPNPEP1 Produits)

Synonymes: anticorps APP1, anticorps SAMP, anticorps XPNPEP, anticorps XPNPEPL, anticorps XPNPEPL1, anticorps D230045I08Rik, anticorps sAMP, anticorps X-prolyl aminopeptidase 1, anticorps X-prolyl aminopeptidase (aminopeptidase P) 1, soluble, anticorps XPNPEP1, anticorps Xpnpep1

Sujet: Aminopeptidase P (AP-P, X-Pro aminopeptidase) has the unique ability to leave the N-terminal amino acid residue from peptides having proline as the penultimate amino acid residue. Biologically active peptides comprise an important and diverse class of extracellular chemical messengers that mediate a wide range of intercellular interactions. Several bioactive peptides including hormones, neuropeptides, neurotransmitters escape non-specific protease degradation by having an Xaa-Pro motif at their amino termini. Due to its cyclic nature, proline confers resistance to such peptide bonds so that aminopeptidases with broad specificity cannot act upon such peptides. There are a limited number of peptidases that act on peptide bonds involving a proline residue, such as dipeptidyl peptidase II (DPPII) and dipeptidyl peptidase W (DPPIV), and prolidase (which cleaves the Xaa-Pro bond only in dipeptides), or endopeptidases such as prolyl endopeptidase (which cleaves on the carbonyl side of proline residues within a protein or peptide). However, none of these enzymes have been reported to hydrolyze Xaa-Pro bonds located at the N-terminus of peptides and proteins. Therefore, role of AP-P is crucial in this respect. AP-P activity is ubiquitous and has been found in a wide range of organisms including bacteria, yeast and vertebrates. Mammalian AP-Ps exist in membrane-bound and cytosolic forms, which represent two distinct gene products. The cytosolic (soluble) form of aminopeptidase P is found in human leukocytes and rat brain.Synonyms: Aminoacylproline aminopeptidase, Cytosolic aminopeptidase P, Soluble aminopeptidase P, X-Pro aminopeptidase 1, X-prolyl aminopeptidase 1 soluble, XPNPEPL, XPNPEPL1, Xaa-Pro aminopeptidase 1

ID gène: 7511

NCBI Accession: NP_065116

UniProt: Q9NQW7