PNPLA3 anticorps (Internal Region)

N° du produit ABIN361184

L’anticorps Chèvre Polyclonal anti-PNPLA3 a été validé pour WB, ELISA et IHC. Il convient pour détecter PNPLA3 dans des échantillons de Humain et Souris. Il y a 4+ publications disponibles.

Aperçu rapide pour PNPLA3 anticorps (Internal Region) (ABIN361184)

Antigène: PNPLA3 (Patatin-Like phospholipase Domain Containing 3 (PNPLA3))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PNPLA3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Classe de qualité: Verified

Détail du produit anti-PNPLA3 anticorps

Épitope: Internal Region

Fonction: Pnpla3 / Adiponutrin (mouse)

Séquence: EHDICPKVKS TN

Specificité: This antibody is raised against a peptide derived from the Mouse protein sequence and this peptide differs from Human only in position 2.

Réactivité croisée: Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence EHDICPKVKSTN, from the internal region of the protein sequence according to NP_473429.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 8 μg/mL.

Western Blot: Approx 45 kDa band observed in Mouse Adrenal Gland lysates, and also in preliminary testing of Mouse and Human Kidney and Rat Adrenal Gland lysate (calculated MW of 42.6Da according to Mouse NP_473429.2). Recommended concentration: 0.01-0.03

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-PNPLA3 (ABIN361184)

Yang, Mottillo, Mladenovic-Lucas, Zhou, Granneman: "Dynamic interactions of ABHD5 with PNPLA3 regulate triacylglycerol metabolism in brown adipocytes." dans: Nature metabolism, Vol. 1, Issue 5, pp. 560-569, (2019) (PubMed).

Hao, Ito, Huang, Sae-tan, Lambert, Ross: "Shifts in dietary carbohydrate-lipid exposure regulate expression of the non-alcoholic fatty liver disease-associated gene PNPLA3/adiponutrin in mouse liver and HepG2 human liver cells." dans: Metabolism: clinical and experimental, Vol. 63, Issue 10, pp. 1352-62, (2014) (PubMed).

Kumari, Schoiswohl, Chitraju, Paar, Cornaciu, Rangrez, Wongsiriroj, Nagy, Ivanova, Scott, Knittelfelder, Rechberger, Birner-Gruenberger, Eder, Brown, Haemmerle, Oberer, Lass, Kershaw, Zimmermann et al.: "Adiponutrin functions as a nutritionally regulated lysophosphatidic acid acyltransferase. ..." dans: Cell metabolism, Vol. 15, Issue 5, pp. 691-702, (2012) (PubMed).

Gustavsson, Soga, Wahlström, Vesterlund, Azimi, Norstedt, Tollet-Egnell: "Sex-dependent hepatic transcripts and metabolites in the development of glucose intolerance and insulin resistance in Zucker diabetic fatty rats." dans: Journal of molecular endocrinology, Vol. 47, Issue 2, pp. 129-43, (2011) (PubMed).

Détails sur PNPLA3

Antigène: PNPLA3 (Patatin-Like phospholipase Domain Containing 3 (PNPLA3))

Autre désignation: Pnpla3

Sujet: Pnpla3, patatin-like phospholipase domain containing 3, Adpn, adiponutrin

Poids moléculaire: 42.6kDa

ID gène: 80339, 116939, 362972

NCBI Accession: NP_473429