NEFH anticorps

N° du produit ABIN361350

Cet anticorps anti-NEFH est un anticorps Souris Monoclonal détectant NEFH dans WB et IF. Adapté pour Boeuf (Vache).

Aperçu rapide pour NEFH anticorps (ABIN361350)

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivité: Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEFH est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: NAP4

Détail du produit anti-NEFH anticorps

Specificité: Specific for the ~200k Neurofilament H protein. It recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.

Réactivité croisée: Poulet, Mammifères

Purification: Protein G purified culture supernatant

Immunogène: Purified bovine NF-H

Isotype: IgG1

Information d'application

Indications d'application: Recommended Dilution: WB: 1:5,000 IF: 1:500 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: total IgG fraction + 10 mM Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur NEFH

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Autre désignation: NEFH

Sujet: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (2) and Alzheimer's disease (3). Anti-Neurofilament H Western blot of rat cortex lysate showing specific immunolableing of the ~ 200k NF-H protein.

Poids moléculaire: '200 kDa

ID gène: 4744

UniProt: P12036