Nuclei anticorps

N° du produit ABIN361360

Cet anticorps Souris Monoclonal détecte spécifiquement dans IHC, ICC et ELISA. Il présente une réactivité envers Humain et a été mentionné dans 4+ publications.

Aperçu rapide pour Nuclei anticorps (ABIN361360)

Antigène: Nuclei

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISA

Clone: 235-1

Détail du produit

Specificité: Specific for endogenous levels of human nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. With immunoprecipitation, the antibody reacts with a ~80 kDa and ~70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4 % Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.

Réactivité croisée: Humain

Attributs du produit: Our Anti-Nuclei Antibody primary antibody is mouse monoclonal. It detects human Nuclei and is concentrated tissue culture supernatant. It is great for use in ELISA, IHC, ICC.

Purification: Concentrated tissue ulture supernatant.

Immunogène: Human cell homogenate.

Isotype: IgG1

Information d'application

Indications d'application: 1:100

Commentaires:

Normally ABIN361360 should be fix with 10% buffered formalin overnight at room temperature instead of 4% paraformaldehyde. 1.Use 10% buffered formalin overnight instead of 4% paraformaldehyde to fix the tissues 2.Quench the endogenous peroxidase activity with 3% H2O2 instead of 0.5% H2O2 3.Block non-specific binding sites with 3% horse serum instead of goat serum.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Concentrated tissue culture supernatent

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Date de péremption: 12 months

Référence

Subramanian, Sidhom, Emani, Vernon, Sahakian, Zhou, Kost-Alimova, Slyper, Waldman, Dionne, Nguyen, Weins, Marshall, Rosenblatt-Rosen, Regev, Greka: "Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation." dans: Nature communications, Vol. 10, Issue 1, pp. 5462, (2019) (PubMed).

van den Berg, Ritsma, Avramut, Wiersma, van den Berg, Leuning, Lievers, Koning, Vanslambrouck, Koster, Howden, Takasato, Little, Rabelink: "Renal Subcapsular Transplantation of PSC-Derived Kidney Organoids Induces Neo-vasculogenesis and Significant Glomerular and Tubular Maturation In Vivo." dans: Stem cell reports, Vol. 10, Issue 3, pp. 751-765, (2018) (PubMed).

Balseanu, Buga, Catalin, Wagner, Boltze, Zagrean, Reymann, Schaebitz, Popa-Wagner: "Multimodal Approaches for Regenerative Stroke Therapies: Combination of Granulocyte Colony-Stimulating Factor with Bone Marrow Mesenchymal Stem Cells is Not Superior to G-CSF Alone." dans: Frontiers in aging neuroscience, Vol. 6, pp. 130, (2014) (PubMed).

Kim, Godarova, Seedle, Kim, Ince, Wells, Driscoll, Godar: "Rb suppresses collective invasion, circulation and metastasis of breast cancer cells in CD44-dependent manner." dans: PLoS ONE, Vol. 8, Issue 12, pp. e80590, (2014) (PubMed).

Détail du antigène

Antigène: Nuclei

Sujet:

Synonyms: HNA antibody, Human Nuclear Antigen antibody, HuNu antibody

Description Logic: Our Anti-Nuclei Antibody primary antibody is mouse monoclonal. It detects human Nuclei and is concentrated tissue culture supernatant. It is great for use in ELISA, IHC, ICC.

Gene Name Alternatives: Marker for human cells in xenographic research