Periostin anticorps (C-Term)

N° du produit ABIN361361

Détail du produit anti-Periostin anticorps

Antigène: Periostin (POSTN)

Épitope: C-Term

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Periostin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificité: Specific for the ~93 kDa periostin doublet in mouse lung extract. This antibody does not recognize the single heart form of periostin or the lowest molecular weight form of lung periostin. Consistent with the fact that this antibody is made against an alternatively spliced region of periostin, this antibody recognizes a subset of the forms of periostin recognized on Western blots by the Pan periostin antibody (Catalog number 1622-PERI) and also shows a distinctive staining pattern by immunohistochemistry (data not shown). The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90° C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). The antibody is expected to work on all mammalian , avian species.

Réactivité croisée: Poulet, Humain, Souris

Homologie: most mammalian and avian

Purification: Antigen Affinity Purified

Immunogène: Bacterial fusion protein equivalent to a 188-amino acid polypeptide from the C-terminal region of mouse periostin which is comprised of six small alternatively-spliced exons

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1,000 IHC: 1:100 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μL in PBS

Stock: -20 °C

Détails sur Periostin

Antigène: Periostin (POSTN)

Autre désignation: POSTN (POSTN Produits)

Synonymes: anticorps fa99h07, anticorps pn, anticorps postn, anticorps wu:fa99h07, anticorps wu:fc70f09, anticorps zgc:153873, anticorps LOC100008945, anticorps osf-2, anticorps pdlpostn, anticorps periostin, anticorps OSF-2, anticorps OSF2, anticorps PDLPOSTN, anticorps PN, anticorps RP11-412K4.1, anticorps A630052E07Rik, anticorps AI747096, anticorps Osf2, anticorps PLF, anticorps peri, anticorps Plf, anticorps periostin, anticorps periostin, osteoblast specific factor b, anticorps periostin, osteoblast specific factor, anticorps POSTN, anticorps postnb, anticorps postn, anticorps Postn

Sujet: Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading (Kruzynska-Frejtag A. et al., 2004). Periostin has recently been shown to promote collagen fibrogenesis, inhibit differentiation of progenitor cells into cardiomyocytes and to be essential in maintaining the biomechanical properties of the adult myocardium (Norris et al., 2008) Anti-Periostin Western blot of rat lung lysate showing specific immunolabeling of the ~ 93 kDa periostin protein doublet.

Poids moléculaire: '93 kDa

ID gène: 50706

UniProt: Q62009