GFAP anticorps

N° du produit ABIN361379

L’anticorps Poulet Polyclonal anti-GFAP a été validé pour WB et IF. Il convient pour détecter GFAP dans des échantillons de Boeuf (Vache). Il y a 1 publication disponible.

Aperçu rapide pour GFAP anticorps (ABIN361379)

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivité: Boeuf (Vache)

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp GFAP est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-GFAP anticorps

Specificité: Specific for the ~50kDa GFAP protein. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule. It is expected that the antibody will react with other mammalian tissues.

Réactivité croisée: Rat (Rattus)

Homologie: human, mouse

Purification: Total IgY fraction

Immunogène: Recombinant and purified bovine GFAP

Information d'application

Indications d'application: Recommended Dilution: WB: 1:10,000 IF: 1:1,000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: total IgY fraction in PBS + 10 mM Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-GFAP (ABIN361379)

Levenga, Wong, Milstead, Keller, LaPlante, Hoeffer: "AKT isoforms have distinct hippocampal expression and roles in synaptic plasticity." dans: eLife, Vol. 6, (2018) (PubMed).

Détails sur GFAP

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Autre désignation: GFAP

Sujet: Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques (1). It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers (2). Anti-GFAP Left: Western blot of rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein. Right: Mixed cultures of neurons and glia stained with chicken anti-GFAP (red), and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.

Poids moléculaire: '50 kDa

ID gène: 281189

UniProt: Q28115