GAP43 anticorps (pSer41)

N° du produit ABIN361475

Détail du produit anti-GAP43 anticorps

Antigène: GAP43 (Growth Associated Protein 43 (GAP43))

Épitope: pSer41

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GAP43 est non-conjugé

Application: Western Blotting (WB)

Specificité: Specific for the ~50k Gap-43 protein phosphorylated at Ser41. In some tissues the antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap-43 antibody, that may be a Gap-43 aggregate or oligomer. Immunolableing is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is completely eliminated by treatment with (-Ptase.

Réactivité croisée: Souris, Rat (Rattus)

Homologie: bovine, canine, chicken, finch, human, non-human primates, Xenopus, zebra fish

Purification: Antigen Affinity Purified from Pooled Serum

Immunogène: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser41 conjugated to KLH

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Stock: -20 °C

Références pour anticorps anti-GAP43 (ABIN361475)

Yi, Kim, Park, Yu, Kim: "The effect of systemic injection of cyclosporin A on the phosphorylation of the PKC substrates MARCKS and GAP43 in the rat hippocampus." dans: Neuroscience letters, Vol. 497, Issue 1, pp. 17-21, (2011) (PubMed).

Détails sur GAP43

Antigène: GAP43 (Growth Associated Protein 43 (GAP43))

Autre désignation: GAP43 (GAP43 Produits)

Synonymes: anticorps B-50, anticorps PP46, anticorps Basp2, anticorps GAP-43, anticorps b-50, anticorps gap-43, anticorps gap43b, anticorps pp46, anticorps cb310, anticorps gap, anticorps gap43, anticorps gap43a, anticorps growth associated protein 43, anticorps growth associated protein 43 L homeolog, anticorps growth associated protein 43 S homeolog, anticorps GAP43, anticorps Gap43, anticorps gap43.L, anticorps gap43, anticorps gap43.S

Sujet: Gap-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that Gap-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). Gap-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C (Catalog No. 1609-PKC) modulates the interaction of Gap-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002). Anti-Phospho Ser41 Gap-43 Western blot of rat cortex lysate showing specific immunolabeling of the ~50k Gap-43 protein phosphorylated at Ser41 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the GAP-43 Ser41 antibody. The immunolabeling of GAP-43 is completely eliminated by treatment with (-Ptase.

Poids moléculaire: '50 kDa

ID gène: 29423

UniProt: P07936