ALOX5 anticorps (pSer523)

N° du produit ABIN361506

Cet anticorps anti-ALOX5 est un anticorps Lapin Polyclonal détectant ALOX5 dans WB. Adapté pour Humain.

Aperçu rapide pour ALOX5 anticorps (pSer523) (ABIN361506)

Antigène: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ALOX5 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-ALOX5 anticorps

Épitope: pSer523

Specificité: Specific for the ~80k 5-LO phosphorylated at Ser523 in Western blots.

Réactivité croisée: Humain, Rat (Rattus)

Homologie: non human primates

Purification: Antigen Affinity Purified from Pooled Serum

Immunogène: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser523 conjugated to KLH

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Stock: -20 °C

Détails sur ALOX5

Antigène: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Autre désignation: ALOX5

Sujet: The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis. Anti-Phospho Ser523 5-Lipoxygenase Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of 5-LO phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the 5-LO Ser523 antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Poids moléculaire: '80 kDa

ID gène: 240

UniProt: P09917