SOD2 anticorps

N° du produit ABIN361656

Détail du produit anti-SOD2 anticorps

Antigène: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SOD2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: Detects ~25 kDa.

Réactivité croisée: Poulet, Boeuf (Vache), Chien, Drosophila melanogaster, Cobaye, Hamster, Humain, Invertébré, Singe, Souris, Porc, Lapin, Rat, Sea Squirt, Mouton, Écureuil, Xenopus laevis

Purification: Protein A Purified

Immunogène: Recombinant Rat Mn SOD Protein

Information d'application

Indications d'application:

• WB (1:5000)
• IHC (1:100)
• ICC/IF (1:120)
• optimal dilutions for assays should be determined by the user.

Commentaires:

0.5 μg/ml of ABIN361655 was sufficient for detection of Mn SOD in 20 μg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Références pour anticorps anti-SOD2 (ABIN361656)

Allan, Storey: "Expression of NF-?B and downstream antioxidant genes in skeletal muscle of hibernating ground squirrels, Spermophilus tridecemlineatus." dans: Cell biochemistry and function, Vol. 30, Issue 2, pp. 166-74, (2012) (PubMed).

Ishii, Kunihiro, Tanaka, Hatano, Nishikata: "Cytosolic subunits of ATP synthase are localized to the cortical endoplasmic reticulum-rich domain of the ascidian egg myoplasm." dans: Development, growth & differentiation, Vol. 54, Issue 8, pp. 753-66, (2012) (PubMed).

Wu, Hsiao, Chien, Lai: "Ischemic conditioning by short periods of reperfusion attenuates renal ischemia/reperfusion induced apoptosis and autophagy in the rat." dans: Journal of biomedical science, Vol. 16, pp. 19, (2009) (PubMed).

Détails sur SOD2

Antigène: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Autre désignation: SOD2 (SOD2 Produits)

Synonymes: anticorps LOC100101896, anticorps MNSOD, anticorps GB14346, anticorps sod2, anticorps MGC88869, anticorps Sod2, anticorps CG8905, anticorps Dmel\\CG8905, anticorps Mito SOD, anticorps Mn SOD, anticorps Mn-SOD, anticorps Mn-SOD2, anticorps MnSOD, anticorps MnSODII, anticorps Mn[2+]SOD, anticorps SOD, anticorps SOD-2, anticorps SOD2, anticorps Sod-2, anticorps dSOD2, anticorps mitSOD2, anticorps mnSOD, anticorps IPOB, anticorps MVCD6, anticorps cb463, anticorps wu:fj33b01, anticorps zgc:73051, anticorps MnSOD, anticorps manganese superoxide dismutase, anticorps superoxide dismutase 2, mitochondrial, anticorps superoxide dismutase 2, anticorps Mn superoxide dismutase, anticorps Superoxide dismutase 2 (Mn), anticorps superoxide dismutase 2 L homeolog, anticorps BDBG_07234, anticorps LOC100101896, anticorps MNSOD, anticorps Sod2, anticorps sod2, anticorps LbMnSOD4, anticorps SOD2, anticorps LOC100282741, anticorps SOD-2, anticorps sod2.L

Sujet: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).

ID gène: 24787

NCBI Accession: NP_058747

UniProt: P07895

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling