FKBP4 anticorps

N° du produit ABIN361687

Cet anticorps anti-FKBP4 est un anticorps Souris Monoclonal détectant FKBP4 dans WB, IHC, IP, IF et ICC. Adapté pour Humain.

Aperçu rapide pour FKBP4 anticorps (ABIN361687)

Antigène: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FKBP4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: Hi52C

Détail du produit anti-FKBP4 anticorps

Specificité: Detects ~52 kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.

Réactivité croisée: Chien, Hamster, Humain, Souris, Rat

Purification: Protein G Purified

Immunogène: Synthetic peptide corresponding to the residues of human FKBP52

Isotype: IgG2a

Information d'application

Indications d'application:

• WB (1:2000)
• IHC (1:250)
• ICC/IF (1:1000)
• IP (5 μg)
• optimal dilutions for assays should be determined by the user.

Commentaires:

0.5 μg/ml was sufficient for detection of FKBP52 in 20 μg total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Détails sur FKBP4

Antigène: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Autre désignation: FKBP52

Sujet: HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).

ID gène: 2288

NCBI Accession: NP_002005

UniProt: Q02790

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway