SOD3 anticorps

N° du produit ABIN361742

Cet anticorps Souris Monoclonal détecte spécifiquement SOD3 dans WB, IHC, ELISA, ICC et IF. Il présente une réactivité envers Humain et a été mentionné dans 2+ publications.

Aperçu rapide pour SOD3 anticorps (ABIN361742)

Antigène: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SOD3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 4GG11G6

Détail du produit anti-SOD3 anticorps

Specificité: Detects extracellular SOD ~35 kDa.

Réactivité croisée: Cobaye, Humain, Souris, Rat

Purification: Protein G Purified

Immunogène: Human extracellular SOD purified from aortas

Isotype: IgG1 kappa

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN361741 was sufficient for detection of EC-SOD in 20 μg of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Références pour anticorps anti-SOD3 (ABIN361742)

Konior, Klemenska, Brudek, Podolecka, Czarnowska, Beręsewicz: "Seasonal superoxide overproduction and endothelial activation in guinea-pig heart; seasonal oxidative stress in rats and humans." dans: Journal of molecular and cellular cardiology, Vol. 50, Issue 4, pp. 686-94, (2011) (PubMed).

Tollefson, Oberley-Deegan, Butterfield, Nicks, Weaver, Remigio, Decsesznak, Chu, Bratton, Riches, Bowler: "Endogenous enzymes (NOX and ECSOD) regulate smoke-induced oxidative stress." dans: Free radical biology & medicine, Vol. 49, Issue 12, pp. 1937-46, (2010) (PubMed).

Détails sur SOD3

Antigène: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Autre désignation: SOD3

Sujet: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).

ID gène: 6649

NCBI Accession: NP_003093

UniProt: P08294