JunB anticorps (pSer259)

N° du produit ABIN361925

Il existe 1 publication pour ce produit. L’anticorps anti-JunB Polyclonal Lapin est utilisé pour la détection de JunB dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB et IHC.

Aperçu rapide pour JunB anticorps (pSer259) (ABIN361925)

Antigène: JunB (JUNB) (Jun B Proto-Oncogene (JUNB))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp JunB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-JunB anticorps

Épitope: pSer259

Specificité: The antibody detects endogenous level of JunB only when phosphorylated at serine 259.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pSer259 (P-V-S (p) -P-I) derived from Human JunB. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-JunB (ABIN361925)

Vartanian, Masri, Martin, Cloninger, Holmes, Artinian, Funk, Ruegg, Gera: "AP-1 regulates cyclin D1 and c-MYC transcription in an AKT-dependent manner in response to mTOR inhibition: role of AIP4/Itch-mediated JUNB degradation." dans: Molecular cancer research : MCR, Vol. 9, Issue 1, pp. 115-30, (2011) (PubMed).

Détails sur JunB

Antigène: JunB (JUNB) (Jun B Proto-Oncogene (JUNB))

Autre désignation: JunB

Sujet: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.

Poids moléculaire: 43 kDa

NCBI Accession: NP_002220

UniProt: P17275