Cité dans 1 publication.
Cet anticorps anti-Ezrin Polyclonal Lapin (ABIN362230) détecte spécifiquement Ezrin dans WB.
L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.
The antibody detects endogenous level of Ezrin only when phosphorylated at threonine 567.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogène
Peptide sequence around phosphorylation site of pThr567 (Y-K-T (p) -L-R) derived from Human Ezrin. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
EZR
Reactivité: Humain, Souris, Rat
WB, ELISA, IF, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western blotting: 1:500-1:1000
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Cui, Li, Zhang, Han: "Expression of Ezrin and phosphorylated Ezrin (pEzrin) in pancreatic ductal adenocarcinoma." dans: Cancer investigation, Vol. 28, Issue 3, pp. 242-7, (2010) (PubMed).
Antigène
Ezrin (EZR)
Autre désignation
Ezrin
Sujet
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.