EIF4B anticorps (pSer422)

N° du produit ABIN362447

L’anticorps anti-EIF4B Polyclonal Lapin est utilisé pour la détection de EIF4B dans des échantillons de Humain et Souris. Il a été validé pour WB et IHC.

Aperçu rapide pour EIF4B anticorps (pSer422) (ABIN362447)

Antigène: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF4B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-EIF4B anticorps

Épitope: pSer422

Specificité: The antibody detects endogenous level of eIF4B only when phosphorylated at Serine 422.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pSer422 (T-G-S (p) -E-S) derived from Human eIF4B. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Détails sur EIF4B

Antigène: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Autre désignation: eIF4B

Sujet: Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Poids moléculaire: 80 kDa

NCBI Accession: NP_001408

UniProt: P23588