OCT4 anticorps (AA 100-200)

N° du produit ABIN363517

Cet anticorps Lapin Polyclonal détecte spécifiquement OCT4 dans WB, IF, ICC et FACS. Il présente une réactivité envers Humain, Souris et Singe.

Aperçu rapide pour OCT4 anticorps (AA 100-200) (ABIN363517)

Antigène: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivité: Humain, Souris, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp OCT4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-OCT4 anticorps

Épitope: AA 100-200

Specificité: Reacts with Oct-4 protein from human, mouse, rat and primate

Réactivité croisée: Boeuf (Vache), Porc

Réactivité croisée (Details): Predicted to cross react with bovine (93 % ), feline (93 %), porcine (93 %).

Purification: Purified

Immunogène: Synthetic peptide made to an internal portion of the human OCT4 protein sequence (between residues 100-200)

Information d'application

Indications d'application:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
- WB : 2 μg/mL - FC: 1:50-1:200 -ICC/IF, 1:50-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.85 mg/mL

Buffer: Tris-glycine, NaCl 150 mM buffer, Sodium azide 0.05 %

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C/-80 °C

Stockage commentaire: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Détails sur OCT4

Antigène: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Autre désignation: Octamer-Binding Protein 4 (Oct-4)

Sujet: Stem cells are crucial ingredients in the progressive restriction of cellular developmental capacity during mammalian embryogenesis. Until recently, only embryonic stem cells (ESC)had been shown to exhibit the potential to differentiate into cells of the all three germ layers. However, adult stem cells (ASC) can regain pluripotency (potential to differentiate into various lineages) if transferred from one stem-cell microenvironment into another.The transcription factor OCT4, expressed in ESCs and germ cells, is strongly implicated in the process of maintaining as well as regaining stem-cell pluripotency and functions as a key regulator of mammalian germline development.

UniProt: Q01860

Pathways: Stem Cell Maintenance