PIK3C3 anticorps (AA 700-850)

N° du produit ABIN363642

Cet anticorps anti-PIK3C3 est un anticorps Lapin Polyclonal détectant PIK3C3 dans WB, IF et ICC. Adapté pour Humain, Souris, Rat et Porc.

Aperçu rapide pour PIK3C3 anticorps (AA 700-850) (ABIN363642)

Antigène: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Reactivité: Humain, Souris, Rat, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PIK3C3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-PIK3C3 anticorps

Épitope: AA 700-850

Specificité: Reacts with human VPS34

Réactivité croisée: Souris, Porc

Réactivité croisée (Details): Cross reacts with proteins from rat, mouse and porcine. The immunogen sequence has 87 % homology with Xenopus protein. Not tested in other species

Purification: Antigen affinity purified

Immunogène: Synthetic peptide made to an internal portion of the human protein (within residues 700-850)

Information d'application

Indications d'application:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
- WB : 1/1 000 - ICC/IF: 1/100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, glycerol 50 % , BSA 0.1 %, Sodium azide 0.05 %

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Short term storage at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Date de péremption: 6 months

Détails sur PIK3C3

Antigène: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Autre désignation: VPS34

Sujet: VPS34, also known as phosphoinositide-3-kinase p100 subunit or PIK3C3 (phosphoinositide-3-kinase, class III), is a member of the PI3/PI4-kinase family. It is ubiquitously expressed with predominant expression in skeletal muscle and is believed to participate in endosome to lysosome transport, multivesicular body formation, autophagy and retrograde endosome to Golgi transport. VPS34 is the catalytic subunit of class III PI3Ks and forms a heterodimer with p150, a regulatory subunit of class 3 PI3Ks. It exclusively phosphorylates phosphatidylinositol to produce PtdIns3P. The activity of VPS34 is enhanced by manganese and can be regulated by nutrients, suggesting an important role of VPS34 in the regulation of mTOR protein synthesis. VPS34 also forms a complex with Beclin1 that promotes autophagy and tumor supression.

Pathways: AMPK Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Toll-Like Receptors Cascades, Autophagy