HSPB6 anticorps

N° du produit ABIN364293

Cet anticorps anti-HSPB6 est un anticorps Lapin Polyclonal détectant HSPB6 dans WB, IHC, IP et IHC (p). Adapté pour Humain, Rat, Souris, Boeuf (Vache) et Lapin.

Aperçu rapide pour HSPB6 anticorps (ABIN364293)

Antigène: HSPB6 (Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6))

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HSPB6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-HSPB6 anticorps

Marque: IHC-plus™

Specificité: Recognizes HSP20, Mr ~20kD. Species cross-reactivity: Human, mouse, rabbit, rat and bovine.

Purification: Immunoaffinity purified

Immunogène: Native HSP20 purified from human skeletal muscle.

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot Analysis: 1 μg/mL detected HSP20 in RIPA lysates from rabbit skeletal muscle and mouse heart cytosol. Immunoprecipitation: Reported to immunoprecipitate HSP20 from rat heart extract and lysates of bovine carotid artery smooth muscle.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M PBS,  pH 7.0, 0.1 % sodium azide, 30 mg/mL BSA, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur HSPB6

Antigène: HSPB6 (Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6))

Autre désignation: HSPB6 / HSP20

Sujet: Name/Gene ID: HSPB6

Synonyms: HSPB6, Heat shock protein beta-6, Hsp20

ID gène: 126393

UniProt: O14558