MHC Class I H-2Db anticorps

N° du produit ABIN371165

L’anticorps anti-MHC Class I H-2Db Monoclonal Souris est utilisé pour la détection de MHC Class I H-2Db dans des échantillons de Souris. Il a été validé pour FACS.

Aperçu rapide pour MHC Class I H-2Db anticorps (ABIN371165)

Antigène: MHC Class I H-2Db

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS)

Clone: 28-14-8

Détail du produit

Specificité: This antibody is specific to an epitope in the alpha 3 domain of H-2Db. It binds to the alpha 3 domain of H-2Db in the presence or absence of beta 2 microglobulin. (Ref.7,8) It cross reacts with the alpha 3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, (Ref.9-11) but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells. (Ref.12). Customer feedback: Clone 28-14-8 does not cross-react with human cells.

Attributs du produit: Synonyms: H-2D(B), H2-D1, H-2 class I histocompatibility antigen, D-B alpha chain

niveau d'endotoxine: Low Endotoxin

Immunogène: C3H. SW mouse splenocytes. (Ref. 1,2)

Isotype: IgG2a

Information d'application

Indications d'application: Flow Cytometry: < / = 1 μg/10^6 cells. Immunohistochemistry on Frozen Tissue Sections. Complement-mediated cytotoxicity.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS without preservatives.

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détail du antigène

Antigène: MHC Class I H-2Db

Autre désignation: MHC Class I H-2 Db

Sujet: The 'classical' MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-Microglobulin. (Ref.3,4) These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (Ref.5)Synonyms: D-B alpha chain, H-2 class I histocompatibility antigen, H-2D(B), H2-D1

ID gène: 14964

UniProt: P01899