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Bu-1b anticorps

Cet anticorps anti-Bu-1b est un anticorps Souris Monoclonal détectant Bu-1b dans FACS, IP et IHC (fro). Adapté pour Poulet.
N° du produit ABIN371415

Aperçu rapide pour Bu-1b anticorps (ABIN371415)

Antigène

Bu-1b (Bu-1b (B Cell Surface Alloantigens) (Bu-1b))

Reactivité

Poulet

Hôte

  • 11
Souris

Clonalité

  • 11
Monoclonal

Conjugué

  • 3
  • 3
  • 3
  • 2
Cet anticorp Bu-1b est non-conjugé

Application

  • 11
  • 7
  • 7
  • 4
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

5-11G2
  • Attributs du produit

    Synonyms: B6.2, Bu1b

    Purification

    Purified

    Immunogène

    Bursal cells from 2- to 5-week-old CHA (Bu-1b) strain Chickens.

    Isotype

    IgG1
  • Indications d'application

    Flow Cytometry: < / = 1 μg/10^6 cells. Immunoprecipitation. Immunohistochemistry on Frozen Sections. (Ref.3)
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.

    Date de péremption

    12 months
  • Antigène

    Bu-1b (Bu-1b (B Cell Surface Alloantigens) (Bu-1b))

    Autre désignation

    Bu-1b

    Sujet

    Bu1b is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has no recognizable similarity to known mammalian molecules and therefore represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. Chicken Bu1b, a product of the Bu1b allele, is expressed on the cell surface as a disulfide linked homodimer. In 10 week old H.B15 and H.B2 chickens, Bu1 is found on 85 to 90 % of bursal cells, 2 to 8 % of thymocytes, 15 to 27 % of spleen cells, and 2 to 18 % of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu1 alleles (Bu1 a/b), Bu1a does not exhibit allelic exclusion.Synonyms: B6.2, chicken B-cell marker

    UniProt

    Q90747
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