Fibrillarin anticorps

N° du produit ABIN371824

Cet anticorps Souris Monoclonal détecte spécifiquement Fibrillarin dans WB, IF et IHC (fro). Il présente une réactivité envers Humain, Rat, Drosophila melanogaster, C. elegans, Plantes et Schizosaccharomyces pombe.

Aperçu rapide pour Fibrillarin anticorps (ABIN371824)

Antigène: Fibrillarin (FBL)

Reactivité: Humain, Rat, Drosophila melanogaster, C. elegans, Plantes, Schizosaccharomyces pombe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Fibrillarin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 38F3

Détail du produit anti-Fibrillarin anticorps

Specificité: Specific for the ~34 kDa Fibrillarin /Nop1p protein. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).

Réactivité croisée (Details): Species reactivity (expected):Mammals.
Species reactivity (tested):Human, Rat, Plant, Drosophila, C. elegans and S. pombe.

Attributs du produit: Synonyms: FIB1, FLRN, Fibrillarin, RNU3IP1, NOP1, LOT3, Nucleolar protein 1, Nucleolar Marker, rRNA2'-O-methyltransferase fibrillarin, U3 small nucleolar RNA-associated protein NOP1

Purification: Ig Fraction

Immunogène: Yeast nuclear preparations.

Isotype: IgG1

Information d'application

Indications d'application: Western Blot: 1/1,000. Immunofluorescence: 1/500. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at -20 °C.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Fibrillarin

Antigène: Fibrillarin (FBL)

Autre désignation: FBL

Sujet: Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67 % identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3).Synonyms: FIB1, FLRN, Fibrillarin, LOT3, NOP1, Nucleolar Marker, Nucleolar protein 1, RNU3IP1, U3 small nucleolar RNA-associated protein NOP1, rRNA 2'-O-methyltransferase fibrillarin

ID gène: 851548

UniProt: P15646

Pathways: Ribonucleoside Biosynthetic Process