AIRE anticorps (Internal Region)

N° du produit ABIN372242

L’anticorps anti-AIRE Polyclonal Chèvre est utilisé pour la détection de AIRE dans des échantillons de Humain, Rat et Chien. Il a été validé pour WB, IHC (p) et EIA.

Aperçu rapide pour AIRE anticorps (Internal Region) (ABIN372242)

Antigène: AIRE (Autoimmune Regulator (AIRE))

Reactivité: Humain, Rat, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp AIRE est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-AIRE anticorps

Épitope: Internal Region

Séquence: C-KDVDLSQPRK GRKP

Specificité: This antibody recognize Apeced Protein (AIRE).

Réactivité croisée (Details): Species reactivity (expected):Dog (Canine) and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogène: Synthetic peptide corresponding to the Internal region of Human AIRE.

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/32000. Western Blot: 0.5-1 μg/mL. Immunohistochemistry on Paraffin Sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris Saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur AIRE

Antigène: AIRE (Autoimmune Regulator (AIRE))

Autre désignation: AIRE / APECED

Sujet: AIRE is a transcriptional regulator that is thought to be important in controlling tissue-specific expression of genes in the thymus. The precise function of the protein is not well defined. It contains zinc finger motifs and isoform 1 is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described. The longer AIRE1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE3 includes the same exon 1-8-9 sequences as found in AIRE2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).Synonyms: Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, Autoimmune regulator

ID gène: 326

NCBI Accession: NP_000374

UniProt: O43918

Pathways: Chromatin Binding